Two tones having frequencies of 1000Hz and 1004 hz are presented separately and then together
Pairs of pure tones are presented having intervals slightly greater than an octave, a fifth and a fourth. The mistunigs are always such that the beat frequency is always 4Hz when played together.
If two pure tones have slightly different frequencies f1 and f2 = f1 + delta f1, the phase difference theta 2 - theta 1 changes continuously with time. The amplitude of the resultant tone varies between A1 + A2 and A1 - A2, where A1 and A2 are the individual amplitudes. These slow periodic variations in amplitude at frequency delta f are called beats, or perhaps we should say primary beats, to distinguish them from second-order beats, that will be described in the next paragraph. Beats are easily heard when delta fis less than 10 Hz, and may be perceived up to about 15 Hz.
A sensation of beats also occurs when the frequencies of two tones It and f2 are nearly, but not quite, in a simple ratio. If f2 = 2f + o (mistuned octave), beats are heard at a frequency o. In general, when f2 = (n/m)f1 + o, mo beats occur each second. These are called second-order beats or beats of mistuned consonances, because the relationship f2 = (n/m)f1. where n and m are integers, defines consonant musical intervals. such as a perfect fifth (3/2), a perfect fourth (4/3), a major third (5/4), etc.

Primary beats can be easily understood as an example of linear superposition in the ear. Second-order beats between pure tones are not quite so easy to explain
Helmholtz (1877) adopted an explanation based on combination tones (Dem
34), but an explanation by means of aural harmonics was favored by others, Wegel and Lane (1924). This theory, which explains second-order beats as resulting from primary beats between aural harmonics of f1 and f2, predicts the correct frequency mf2 - nf1 but cannot explain why the aural harmonics are themselves not heard (Lawrence and Yantis, 1957).
An explanation which does not require nonlinear distortion in the ear is favored by Plomp (1966) and others. According to this theory, the ear recognize variations in waveform, probably as a periodicity in nerve impulses evoked when the displacement of the basilar membrane exceeds a critical value. This implies that simple tones can interfere over much larger frequency differences than the critical bandwidth and also that the ear can detect changing phase (even though it is a poor detector of phase in the steady state).
Beats of mistuned consonances have long been used by piano tuners, for example, to tune fifths, fourths, and even octaves on the piano. Violinists also make use of them in tuning their instruments. In the case of musical tones, however, primary beats between harmonics occur at the same rate as second-order beats, and the two type cannot be distinguished.
In the first example, pure tones having frequencies of 1000 and 1004 Hz are presented together, giving rise to primary beats at a 4-Hz rate. In the next example, tones with frequencies of 2004 Hz, 1502 Hz, and 1334.67 are combined with a 1000-Hz tone to give secondary beats at a 4-Hz rate (n/m=2/1, 3/2, and 4/3, respectively).
It is instructive to compare the apparent strengths of the beats in each case.